FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934480
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27320.1412265828543071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46800.049.982231
ATTCGCG400.00259541234.50368530
GTATCAA99650.032.787211
GTGGTAT12800.028.8406871
TGGTATC13250.028.120852
TATCAAC126900.026.1537512
ATCAACG132000.025.325573
TCAACGC134050.024.8867974
CAACGCA139350.024.113565
AACGCAG143950.023.390936
GCGAACT4650.022.25813928
GTACTTT48750.020.38055414
TACATGC5100.020.2941862
CGTTTAT2051.7389539E-920.19936833
ACGCAGA166900.020.1512267
CGCAGAG167500.020.0594848
GCAGAGT169600.019.8319599
AGAGTAC174400.019.3652511
CAGAGTA174950.019.2846510
TAAGACT5200.019.2389589
Produced by FastQC (version 0.11.5)