FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3980250
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43220.10858614408642672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56950.043.2152441
GTATCAA165050.043.1235351
ATCAACG233650.030.8745213
TCAACGC237850.030.3732284
CAACGCA242150.029.9478515
TATCAAC241500.029.8667852
AACGCAG248250.029.2540326
GCGAACT10000.026.2192428
GTACTTT101250.025.24879314
CGCAGAG287950.025.2311428
ACGCAGA288250.025.1812637
AGAGTAC295800.024.52656411
GCAGAGT298300.024.4713559
CAGAGTA300150.024.24006810
CGAACTA11250.023.91930829
GAGTACT109850.023.33491312
AGTACTT109900.023.29290413
TACTTTT114800.022.35936215
TACATGC13050.022.2140672
TAAGACT12000.022.1363039
Produced by FastQC (version 0.11.5)