FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203814
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42350.19216685255652247No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23010.1044099002910409No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCAACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG22880.10382001384871863RNA PCR Primer, Index 36 (96% over 30bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45800.047.908411
TCGTATG4100.046.28164740
CTCGTAT4250.044.64817439
GTATCAA133250.043.0053521
TCTCGTA4500.042.93440638
TATGCCG4550.042.4568243
ATCTCGT4900.038.72545637
CGTATGC5300.036.44960841
CCGTCTT5450.035.44962347
ATCAACG187450.030.4942343
TATCAAC189050.030.274022
TCAACGC191050.029.8647774
CGTCTTC6600.029.27279548
CAACGCA196250.029.1086145
AACGCAG201400.028.3814056
TATCTCG6850.027.70393836
GCCGTCT7450.025.93000446
ACGCAGA233900.024.4673527
CGCAGAG235850.024.2650578
GCAGAGT243750.023.4220129
Produced by FastQC (version 0.11.5)