FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203814
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50730.23019184014621924No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA117400.042.1471561
ATACATG8550.041.1643521
GGTATCA41100.034.085641
TATCAAC173600.030.0661542
ATCAACG176350.029.4988233
TCAACGC180450.028.8668164
AACGCAG184000.028.4042636
CAACGCA184150.028.3992125
CGTCGAT809.56586E-525.8749553
GTACTTT67950.025.69031514
TCTGTCG4050.024.7019738
CGCAGAG214050.024.4327668
ACGCAGA214050.024.4166497
GCGAACT5450.024.05390528
GCAGAGT219050.023.9380639
AGTACTT73650.023.74782813
AGAGTAC221350.023.70491211
GAGTACT74200.023.66478512
CAGAGTA223650.023.52283310
CCGAAAT7600.023.15074547
Produced by FastQC (version 0.11.5)