FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345756
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50330.21455769483271067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28820.12286017812594319No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43500.049.3305931
GTATCAA137200.044.784221
CTCGTAT3150.040.5243139
TCGTATG3100.040.06415640
TCTCGTA3450.036.00044338
CGTATGC3600.034.4982241
TATGCCG3700.032.63345343
ATCTCGT3900.031.84654837
ATCAACG196950.031.180893
TATCAAC197850.031.1597842
TCAACGC200350.030.6689594
CAACGCA205300.029.9631085
AACGCAG211250.029.1355086
TATCGCG500.00773445627.60151949
ACGCAGA246200.024.984957
CGCAGAG247300.024.8738178
CCGTCTT5100.024.3542847
TATCTCG5100.024.3542836
CGTTCGG851.4444301E-424.35168624
GCAGAGT254750.024.1328569
Produced by FastQC (version 0.11.5)