FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345756
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50010.2131935290797508No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA117800.042.700351
ATACATG9300.040.4354971
GGTATCA41250.039.8945581
TATCAAC179450.029.6257342
ATCAACG180250.029.4368323
TCAACGC184800.028.7307284
CAACGCA188900.028.2349825
AACGCAG189500.028.1995966
ACGCAGA222550.023.9952667
CGCAGAG222950.023.9063078
CCGAACG1301.8498577E-723.88360413
TCTGTCG3500.023.655648
GCAGAGT227100.023.5150179
AGAGTAC231600.023.22246211
CAGAGTA232050.023.16256110
GTACTTT77150.022.6263914
CCGAAAT6400.022.10062847
AGTACTT80500.021.64194113
TTGCGAT800.00290031721.56250649
GAGTACA170950.021.25005112
Produced by FastQC (version 0.11.5)