FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4622275
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG110890.23990351071712523No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA170000.039.043831
GGTATCA60500.037.1975441
ATCAACG234950.028.9421753
TCAACGC238850.028.4407124
TATCAAC242300.028.0572812
CAACGCA243100.028.0002635
AACGCAG244700.027.8453776
ATACATG10300.027.1437511
GCGAACT13250.024.21461928
CGCAGAG284350.023.9256788
ACGCAGA287650.023.6874337
GCAGAGT293950.023.166779
CGGTACG1202.4581568E-623.00253735
CCGAAAT14000.022.91740847
AGAGTAC303600.022.45313311
CAGAGTA306250.022.22505210
TACATGC15900.021.9208322
CGAACTA14850.021.83842829
TCGCCCC14950.021.69469838
CACGTAA13200.021.43047161
Produced by FastQC (version 0.11.5)