FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4210868
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG159730.3793279675354345RNA PCR Primer, Index 41 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG83270.19775020257106138RNA PCR Primer, Index 41 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG58600.1391637068651879RNA PCR Primer, Index 41 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51630.12261130009299745No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA280450.033.182621
TCGTATG41400.031.0851140
CGTATGC41600.030.76503841
TATGCCG41650.030.72956343
ATCTCGT41050.030.59479937
CCGTCTT41700.030.52689747
ATACATG8950.030.4571481
CTCGTAT41400.030.41876439
TCTCGTA41900.030.05577538
GCCGTCT42450.029.98719646
CATCTCG43750.028.78723536
CGTCTTC44650.028.51067748
CGACATC44450.028.33019433
GACATCT46300.027.27628934
GGTATCA134900.026.8828891
ATACGCA6300.026.8358236
GTCGACA48050.026.71025331
TCGACAT48100.026.68280432
AGTCGAC50350.025.55833830
ATGCCGT50400.025.39396944
Produced by FastQC (version 0.11.5)