FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4210868
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG395160.9384288464991066No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACATG10900.030.3872241
GTATCAA294500.028.7147051
ATACGCA5550.026.1063336
GCGAACT8900.025.19551528
CAATACG6200.025.038624
AACGCAG356600.024.7075186
CCGAAAT10400.023.55352247
TATCAAC389000.022.4542482
ATCAACG392400.022.3830343
TCGCCCC11000.022.2719638
TCAACGC400500.021.9647984
CAACGCA407850.021.6535475
CGCAGAG411600.021.3892158
ACGCAGA414200.021.2801917
TATACCG7700.021.0570096
TCTATCG2800.020.94674531
GCAGAGT423100.020.8891379
AAAGCGA10750.020.85879925
CGAACTA10500.020.69982129
CAGAGTA433900.020.3932910
Produced by FastQC (version 0.11.5)