FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4012605
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74250.1850418867543653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA133550.041.036051
GGTATCA50800.036.9567831
TATCAAC187900.029.4514472
ATCAACG188150.029.2824953
TCAACGC189800.029.0642854
CAACGCA193800.028.553415
AACGCAG195300.028.4220776
ACGCAGA231500.023.9776747
CGCAGAG232300.023.8648038
GCAGAGT239800.023.2622689
AGAGTAC240750.023.07103211
CAGAGTA245150.022.72646110
GTACTTT74050.022.45584314
GAGTACT76800.021.56192212
AGTACTT77900.021.30173913
AGTACAT188600.020.67017413
TACTTTT81300.020.45383515
GAGTACA190950.020.45192312
ATACGCA5300.019.5275346
ACTTTTT87050.019.34105116
Produced by FastQC (version 0.11.5)