FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943136
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36710.18892141363239628No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29950.15413228924789618No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22970.11821097442484725No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20420.10508785797803138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45850.050.952211
GTATCAA94450.030.6895851
GTGGTAT11900.027.8379971
TCGTATG2000.027.6010440
CGACAAC500.00774159227.59606714
GCGAACT5550.027.3502728
CTCGTAT2050.026.92853739
TGGTATC11900.026.678082
TCTCGTA2000.025.87730838
CGAACTA6150.024.68195229
TACATGC6350.023.9107192
TATCAAC121450.023.8100432
ATCAACG122050.023.6911643
TAAGACT6300.023.5442649
ATCTCGT2200.023.52482437
TCAACGC125250.023.0846924
CAACGCA128800.022.4478515
TGTACGG3100.022.25489410
AAAGCGA6900.022.00026325
TCTTACG953.0696302E-421.7863711
Produced by FastQC (version 0.11.5)