FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4593700
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63530.13829810392494069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61150.041.8622631
GTATCAA180000.040.1346441
TCGTATG7650.039.23537440
TATGCCG8250.036.79927443
CGTATGC8550.035.50768341
ATCTCGT8350.034.29348437
CTCGTAT8600.033.29694439
ATCAACG240950.029.8534413
TCAACGC245900.029.2384574
TATCAAC249400.028.8139862
CAACGCA250100.028.733345
AACGCAG257200.027.9398526
GCGAACT13200.027.4426428
TCGAATC10900.026.90548133
CCGTCTT10750.026.31633447
AATCTCG11250.025.76173636
CGAACTA14150.025.60020329
CGAATCT11450.025.3117534
TACATGC16150.024.3524592
GCCGTCT11650.024.28251846
Produced by FastQC (version 0.11.5)