EGAF00005402176 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005402176
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4022149
Sequences flagged as poor quality	0
Sequence length	75
%GC	45

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	15308	0.3805925638259547	RNA PCR Primer, Index 41 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5104	0.12689733771672806	No Hit
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG	4755	0.11822038417771197	RNA PCR Primer, Index 41 (96% over 30bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTATGC	2645	44.083794	41
TATGCCG	2650	43.998978	43
TCGTATG	2640	43.91249	40
CTCGTAT	2620	43.72149	39
ATCTCGT	2670	42.90167	37
TCTCGTA	2735	41.882065	38
CCGTCTT	2760	41.87252	47
GCCGTCT	2805	41.566628	46
CATCTCG	2810	40.89056	36
CGTCTTC	2935	39.72848	48
CGACATC	3045	37.958607	33
GTCGACA	3235	36.260216	31
GACATCT	3280	35.66288	34
GTATCAA	25565	35.088444	1
TCGACAT	3330	35.018555	32
AGTCGAC	3385	34.551487	30
ATGCCGT	3490	33.408966	44
TGCCGTC	3650	32.417015	45
CTTGAAA	3680	31.31843	57
GTATGCC	3890	30.240763	42