FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4022149
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG270180.6717304605075546No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACATG10300.031.4943121
GTATCAA284100.027.8410051
CATCGTT3300.026.1417832
CGGTAAG1607.8216544E-1125.87521428
AACGCAG329000.025.0193276
CAATACG6650.023.8636724
ATACGCA6400.023.1787266
GCGAACT8000.022.8564428
TATCAAC365600.022.4405632
ATCAACG368400.022.3716283
TCAACGC376100.021.8860914
CGCAGAG378600.021.6946628
CAACGCA382150.021.656965
ACGCAGA382200.021.5181967
GTACTTT140600.021.37144914
GCAGAGT387100.021.289589
TCGGTAA1808.598363E-921.08298527
CGTTCGG1808.605639E-921.08167524
AGAGTAC396900.020.84291311
CAGAGTA398250.020.73709310
Produced by FastQC (version 0.11.5)