FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005402178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4025332
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50000.046.3009871
GTATCAA142850.044.295211
TATCAAC197850.032.013352
ATCAACG203850.031.0714783
TCAACGC206450.030.7310624
CAACGCA211500.030.0781065
AACGCAG218400.029.1278366
ACGCAGA255100.024.8832637
CGCAGAG258700.024.5503278
GAGTACT98500.024.51607712
AGAGTAC264200.024.0517111
AGTACTT101650.023.72241813
CAGAGTA268650.023.64076210
GCAGAGT270450.023.5092249
GTACTTT104050.023.14208414
TACTTTT111850.021.65188215
GTTCGCG800.002900585521.5626230
ATAAGCG3450.020.99881422
GAGTACA191100.020.72384512
AGTACAT193300.020.4522913
Produced by FastQC (version 0.11.5)