FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005402206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13673869
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGCAGTATCTCGTAT230780.16877447048819905TruSeq Adapter, Index 2 (97% over 36bp)
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG168520.1232423683450529No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGCAGTATCGCGTAT154410.11292341618893671TruSeq Adapter, Index 2 (97% over 36bp)
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA149060.10901084396815561No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT147330.10774565706311798No Hit

[OK]Adapter Content

Adapter graph