FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005402326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005402326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16067966
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGCAGTATCTCGTAT358290.22298404166401645TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGCAGTATCGCGTAT217140.13513844876196526TruSeq Adapter, Index 2 (97% over 36bp)
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG206280.1283796592549424No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA181600.11301990556863263No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT177430.11042467976344983No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA162100.10088395755878499No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)