FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005403032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12691053
Sequences flagged as poor quality0
Sequence length101
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATGGTGATCTCGTAT2063181.6256964650608583TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATGGTGATCGCGTAT947070.7462501338541412TruSeq Adapter, Index 3 (97% over 36bp)
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG416420.3281209210929936No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA275720.21725541607934348No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT273710.21567162315057703No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA238830.18818769411805308No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC217470.17135693941235608No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG193670.152603570405072No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA193310.1523199059999198No Hit
ACCCGAACCTGGGCTACCACCCTTAATCCCCAGATCGGAAGAGCACACGT157420.12404014071960773No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATGGTGATCTCGTA157310.12395346548470013TruSeq Adapter, Index 3 (97% over 36bp)
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC148640.11712188106061806No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT143600.11315057938848731No Hit
GGGGATTAAGGGTGGTAGCCCAGGTTCGGGTAGATCGGAAGAGCACACGT141980.11187408956530243No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA137100.1080288609621282No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG132880.10470368376839967No Hit
GACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAG132610.10449093546453553No Hit
ACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGG129390.10195371495178532No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC127800.1007008638290298No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)