FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005403040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11511627
Sequences flagged as poor quality0
Sequence length101
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATGGTGATCTCGTAT1683801.462695064737591TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATGGTGATCGCGTAT874490.7596580396498254TruSeq Adapter, Index 3 (97% over 36bp)
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG362330.3147513379299034No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA244050.21200304700630068No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT237600.20640001626181947No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA207490.1802438525848692No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC192340.1670832454873668No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA181890.15800546699437013No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG163400.14194344552685734No Hit
ACCCGAACCTGGGCTACCACCCTTAATCCCCAGATCGGAAGAGCACACGT150350.13060708099732557No Hit
GGGGATTAAGGGTGGTAGCCCAGGTTCGGGTAGATCGGAAGAGCACACGT130140.11305091799795111No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC126020.10947192781698017No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT125690.10918526112772764No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA124980.10856849340236616No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATGGTGATCTCGTA122900.10676162457313809TruSeq Adapter, Index 3 (97% over 36bp)
GACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAG117540.10210546259012734No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG117110.10173192720716194No Hit
ACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGG115530.10035940184649833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)