FastQCFastQC Report
Wed 11 May 2022
EGAF00005403118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62915293
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT7527091.1963847963006389No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG7138191.134571526194752No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG4840140.7693105712787509No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA3635930.577908776487777No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2689430.4274684058135118No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2469760.3925532064199399No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT1944340.30904091951061885No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1819820.2892492291182686No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC1745090.27737135389324186No Hit
GGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCTTGT1369820.21772448870261163No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA1202910.1911951677631065No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC1186550.1885948460893284No Hit
GGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGA1130070.1796176964478255No Hit
CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC1059260.1683628811837529No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCA1001410.15916797844365121No Hit
GTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAG969460.15408972187413958No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC898640.14283331717139106No Hit
CAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTT884990.1406637333787828No Hit
GAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAG846590.13456028886331342No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC836650.13298038681946533No Hit
AGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGC826050.13129558182300763No Hit
CTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATAT812970.12921659603492588No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC783590.12454682520512143No Hit
CGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC781520.1242178113992094No Hit
GTGGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAGAGGGAGCTGACG773770.12298599642538421No Hit
TCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCC735540.11690957236740517No Hit
CCAGAATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCT654560.10403829796993873No Hit
CTGAGAATGAGCCCCGTGTGGTTGGTGCGCGGACACGCACTGCCTGCGTA649700.10326583077344961No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)