FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53728445
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT3267800.6082066957270027No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG2979800.5546038043721533No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA1923170.35794261307953357No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG1732160.32239161211533296No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1319200.24553102178929614No Hit
GTGGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAGAGGGAGCTGACG1224750.22795187912101308No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC848680.157957298038311No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA731850.13621276402099483No Hit
GTGTGGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAGAGGGAGCTGA724540.13485221841056447No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC707180.13162115523723047No Hit
CTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTTGAGAGCT630880.11742011145120615No Hit
GGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAGAGGGAGCTGACGGA630090.11727307574228141No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC622740.1159050852858295No Hit
CTGAGAATGAGCCCCGTGTGGTTGGTGCGCGGACACGCACTGCCTGCGTA608090.11317841043045263No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA560400.10430229276131106No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC559500.10413478372582716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC115650.027.9615754
CAGACCG120400.027.3906943
GACCGCG119700.026.8851835
ACCGCGT119900.026.8586836
TGGAGTC609100.025.1923082
CCGCGTT129050.024.9713757
GGAGTCT646550.023.8054053
GAGTCTT655900.023.360034
CGCGTTC138000.023.3359158
AGTCTTG701450.022.0393035
TACGGAG149350.021.65171434
AATACGG154950.020.98278232
CAATACG155900.020.79828531
GTCTTGG742800.020.6673186
CCCTACG731750.020.47433324
ATACGGA157800.020.40874533
CTAACGC50500.020.38652244
GCGTTCT158850.020.3699479
CCTACGT735850.020.33639725
TACGTTC745850.020.14060227
Produced by FastQC (version 0.11.5)