FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005403153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61266420
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA7943531.2965552744880475No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA4012670.6549542147231713No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC2615760.42694840011869467No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT2397700.3913563090515163No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC2302700.37585026185633175No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG2124030.3466874676209251No Hit
CGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCA1986620.32425919451471136No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1904670.31088318853949687No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1733060.2828727384430166No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1728490.2821268159621535No Hit
CCGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCC1716910.2802367104198352No Hit
TCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1688030.2755228720724991No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1491050.24337149126715746No Hit
GCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAG1477170.24110597616116627No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT1416380.23118373817174234No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC1340790.21884582125085814No Hit
CCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTTT1259130.20551714952497632No Hit
TGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGA1250180.2040563166576405No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1219140.1989899197635507No Hit
CGCGCTGAGAATGAGCCCCGTGTGGTTGGTGCGCGGACACGCACTGCCTG1206250.19688599399148832No Hit
CCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCG1173420.1915274305239314No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG1149350.18759868782931988No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG1142290.18644634369039353No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA1088650.17769113977934406No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1078950.176107890749941No Hit
GACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAG1053210.1719065680677931No Hit
GCCGCGCTGAGAATGAGCCCCGTGTGGTTGGTGCGCGGACACGCACTGCC1048620.17115738115594153No Hit
CCGCGCTGAGAATGAGCCCCGTGTGGTTGGTGCGCGGACACGCACTGCCT982600.16038149446303537No Hit
TCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCC979390.15985755328938756No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA978570.15972371161886073No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA950340.15511596727864954No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC931300.15200822897763572No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC897660.14651745605504612No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC870990.14216433733193484No Hit
CACCCGTTTACCTCTTAACGGTTTCACGCCCTCTTGAACTCTCTCTTCAA869890.14198479362756955No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT830660.13558161224370543No Hit
GCCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCG828100.13516376507718256No Hit
CCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAAT794780.12972522305040837No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA786280.1283378398803129No Hit
TGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTAC784300.1280146612124554No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC780410.12737972938519992No Hit
CGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGG767700.12530518349203365No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC757330.12361257602451717No Hit
CCCGCCGCCGGGTTGAATCCTCCGGGCGGACTGCGCGGACCCCACCCGTT750600.12251409499690043No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT750080.12242921979120046No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC725170.11836337099507366No Hit
GTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGC677570.11059402524253906No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA665180.10857171024518815No Hit
ACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGG661720.10800696368418458No Hit
CCCGTGTGGTTGGTGCGCGGACACGCACTGCCTGCGTAACTAGAGGGAGC653550.10667344362539871No Hit
CCGGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA647060.10561413576964347No Hit
GACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAG627830.10247538537423928No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT616110.10056242881500176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)