FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005403255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12664871
Sequences flagged as poor quality0
Sequence length101
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATGGTGATCTCGTAT1943401.5344806907231823TruSeq Adapter, Index 3 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATGGTGATCGCGTAT942950.7445397588337063TruSeq Adapter, Index 3 (97% over 36bp)
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG421230.33259715002229395No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA276170.21805986022281634No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT270520.21359870147907548No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA238740.1885056705275561No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC216480.1709294946628355No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA192660.1521215652334714No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG189530.1496501622480008No Hit
ACCCGAACCTGGGCTACCACCCTTAATCCCCAGATCGGAAGAGCACACGT159840.12620736523885637No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC146390.11558743867189805No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT144990.11448201880619234No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATGGTGATCTCGTA143640.11341607822140469TruSeq Adapter, Index 3 (97% over 36bp)
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA141300.11156844787443947No Hit
GGGGATTAAGGGTGGTAGCCCAGGTTCGGGTAGATCGGAAGAGCACACGT138040.1089943987585819No Hit
GACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAG136060.10743101923422671No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG131750.10402790521908988No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC130020.10266192209932498No Hit
ACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGG129150.10197498261135071No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG127910.10099589644458282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)