FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25681844
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAACG242550.0140.420272
ATCGCGA170200.0136.985492
CGCGTTT325800.0136.176731
CACGCGA197950.0135.978099
TCACGCG199400.0135.498268
CCGGTCG139950.0134.32064
GCGCAAC260800.0134.27741
ACGCCGA128400.0134.206161
CGTCGAT124150.0133.600727
TCGTTGC420550.0132.248418
TCGCGAG175550.0131.90073
CGGTCGG140950.0131.666995
CCCGTCG126900.0131.106165
TCGTTCG91150.0131.069028
CGACACG112800.0130.977625
AACCGCG136350.0130.293731
TAATCGG207300.0130.195435
CCAGTCG311300.0129.970844
CGCGAGT176700.0129.729394
GATCGAT186050.0129.464661
Produced by FastQC (version 0.11.5)