FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403416

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403416
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26754959
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGAT256350.0130.562151
GTCGTAC171800.0130.134129
GTCGCGT95900.0129.037198
GCCGATA167050.0128.632162
CGCGGTA97150.0128.299961
CGGTCGC95400.0128.041726
TAGCGCG67700.0126.997747
CCGATAG168100.0126.966143
GGTCGCG96850.0126.424137
GCGGTCG97750.0124.593375
CTTCGAC176000.0124.557286
AATCGGT188650.0124.125651
AGGTCCG186350.0123.893351
ATCCGCG44150.0122.9846957
CGTCGAC55300.0122.963926
CGGATCG81250.0122.597394
GATCGTA161050.0122.533311
TCGACAA274100.0122.268578
GACGTCG59650.0121.536692
GATCGAT154950.0121.321711
Produced by FastQC (version 0.11.5)