FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25184944
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG56100.0133.357549
AACGTCG64700.0130.313144
CGCGTTT121900.0130.214521
CGAATCG73400.0129.188694
CGAAGCG96050.0128.684959
CGCTCGA82200.0128.14295
CTCGTAC151100.0127.879321
GCGGTCG91350.0127.687455
CGTACGT81350.0126.811953
GCGACGT57350.0126.026689
AACCGCG69350.0125.717951
CGTCCGA78000.0124.6335456
AGCGCGA88600.0124.532218
GTTACGC93300.0123.464768
CCGCTCG87300.0123.068464
CGGGTCG87650.0122.74272
GACGGCT172600.0122.492921
TTGATCG194350.0122.159589
GTCCGAC79550.0121.840587
GTCGCCG58300.0121.361799
Produced by FastQC (version 0.11.5)