FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403425

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403425
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25184944
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC94100.0123.875325
CACGACG95400.0122.11134
CGACGCT95850.0119.571626
GACGCTC112350.0102.009827
ACACGAC115750.0101.019093
CTACACG117450.099.9467541
TACACGA120200.097.641832
ACGCTCT124300.091.9694758
CGCTCTT144600.079.2072759
TCCGATC118150.018.83630815-19
TTCCGAT128450.017.91340810-14
CTTCCGA132200.017.54785210-14
GCGTACG7950.016.4134122
TCTTCCG144650.016.15779310-14
CGGTACG6851.3096724E-1015.8740974
CCGATCT142000.015.79512515-19
CGCCGTA266300.015.12939160-64
TCGCCGT270950.014.73597260-64
GCCGTAT274550.014.58499460-64
CGTATCA276400.014.33029565-69
Produced by FastQC (version 0.11.5)