FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31773539
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC110550.0119.6740345
CGACGCT111400.0117.589566
CACGACG114150.0116.2173544
ACACGAC136250.097.792323
GACGCTC135950.096.566737
CTACACG140500.096.248731
TACACGA141250.094.998742
ACGCTCT152950.085.73888
CGCTCTT178750.073.321469
ACGCGAC14200.019.3997062
CGCGACT15150.018.183053
TCCGATC143700.017.82793615-19
CGCCGTA430200.017.62179460-64
TCGCCGT433850.017.34321660-64
GCCGTAT438650.017.1369160-64
CGTATCA440250.016.96648665-69
TTCCGAT156250.016.9532910-14
CTTCCGA159950.016.86932210-14
GTCGCCG451400.016.8198860-64
CCGTATC441550.016.30908865-69
Produced by FastQC (version 0.11.5)