FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25087408
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC187250.0127.794715
CACGACG189250.0126.6739964
CGACGCT190450.0124.848126
ACACGAC209150.0115.245453
GACGCTC207300.0114.453477
CTACACG213000.0114.426991
TACACGA217150.0111.366922
ACGCTCT223250.0105.95218
CGCTCTT243950.097.0193949
TTCCGAT234200.020.56601110-14
TCCGATC231300.020.41567215-19
CTTCCGA239600.020.36271310-14
TCTTCCG253950.019.17210610-14
CCGATCT260300.018.35282115-19
CGCCGTA318650.015.68296460-64
CGTATCA322150.015.44998565-69
TCGCCGT324850.015.38364560-64
CGATCTA67200.015.31830315-19
GCCGTAT323950.015.29659860-64
GTCGCCG340350.014.79380260-64
Produced by FastQC (version 0.11.5)