FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24787597
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG50250.0130.565864
CGCGATA73000.0129.004842
CGTACCG47400.0128.622597
CGGCTAG195550.0126.384651
TATCGCG54850.0125.9551246
TAGTCGA147800.0125.711395
TAGGTCG114050.0125.2826469
CTTACCG136400.0125.1147841
ACGTACG62750.0123.499036
TCGCGAG106400.0123.108731
CGTACGT63300.0122.99867
TAACGCG43900.0122.864992
TCGTCGC46450.0118.611696
CGCAACT117250.0118.221042
TCTACGG134150.0116.778718
AATCGCG46500.0116.613349
GATCGAT133900.0116.375211
ATCGTTA165100.0116.182937
GATCCGA99650.0116.106139
CGACCTT164950.0115.3733751
Produced by FastQC (version 0.11.5)