FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25994371
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG183450.0137.992079
GTCCGCG154900.0134.92457
ACGCCGA148650.0134.782551
GACGCGT131700.0134.541751
CGCCGAA141050.0134.509082
GCGTTCG120750.0134.126224
TCGCGCA122350.0133.67583
TGTCGCG104250.0133.582297
TAGCGCG112850.0132.973897
CGGACGT109100.0132.340671
TACTCGC165400.0132.000691
AATCGGT247850.0131.485241
TCCGCGG160050.0131.17188
ACGCGTT133450.0129.185652
CGATCGG99900.0128.875841
CGTAACG113650.0128.848567
CGGGCTA247900.0128.763612
CTAGCGT184600.0128.689276
GCGCGTC116500.0128.68339
CGACACG92300.0128.493165
Produced by FastQC (version 0.11.5)