FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26954912
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTCG130450.0135.433354
ACGTCGT114450.0132.830183
CGCGCAA105050.0131.810944
CGTAACG73150.0131.312267
CACGTCG172200.0131.26438
TCGTACG89400.0130.963652
ATCGCGA66350.0130.56982
CGGACGT134000.0129.942291
CGTACGT90300.0128.694473
GCGCGTA60050.0128.573294
CGATCGG78500.0128.358871
TTGCGCG63100.0128.333372
GTTCGCG71950.0127.859956
TAAACCG170100.0127.107721
GTGCGAA211900.0127.061698
TTCCGCG85050.0125.724937
AACGCCG97750.0125.632019
GGCGACT151450.0124.9573441
TGGTTCG172100.0124.772354
CATCGGG206150.0124.693491
Produced by FastQC (version 0.11.5)