FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26954912
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACG103400.0119.114564
ACGACGC103850.0118.458815
CGACGCT104350.0116.3628546
ACACGAC123100.0100.641263
CTACACG127600.098.532221
GACGCTC125200.097.2724157
TACACGA129550.096.1343462
ACGCTCT138650.087.9408958
CGCTCTT163000.074.891829
TCCGATC131250.018.14930315-19
CTTCCGA145950.017.1960510-14
TTCCGAT143150.017.08674810-14
ACCGACG13550.015.6717451
TCTTCCG161550.015.49064710-14
CGCCGTA273750.015.37966260-64
CCGATCT159200.015.199697515-19
TCGCCGT279650.014.99815860-64
TCGCGAT11150.014.9531579
GCCGTAT283900.014.68682160-64
CGTATCA286350.014.46020165-69
Produced by FastQC (version 0.11.5)