FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27367693
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACG63500.0131.629736
CCGATCA220450.0128.845783
ACGTCGG142700.0128.781179
CGTCGCG63750.0128.61186
TCGTCGC65300.0125.670255
CGACACG87500.0125.351665
GACGGCT205550.0124.383151
CACGTCG148550.0123.90498
CGGACGT85100.0123.693071
TCCCGAT205700.0123.404641
TTGATCG222600.0123.362529
CTCGCGT96200.0123.2135853
GCCGATC161600.0122.921172
GCTACCG175150.0122.8074952
GTCGACG37850.0122.386478
CGCTGGA303400.0122.044351
CGCGCAA54850.0121.99474
CGCGTTC94350.0121.552485
GGCCGAT168850.0121.264311
CCGGTAG138350.0120.700221
Produced by FastQC (version 0.11.5)