FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26039350
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAACG247850.0140.119582
ATCGCGA177500.0136.835482
CGCGTTT342550.0136.24841
ACGCCGA126450.0135.459381
CACGCGA204600.0134.252669
TCACGCG205650.0134.20178
GCGCAAC265000.0133.845951
CGTCGAT125500.0133.782327
TCGTTGC425100.0133.272878
CCGGTCG143000.0132.633734
CTAATCG205100.0130.97094
TCGTTCG94750.0130.834058
CGGTCGG142450.0130.702795
TCGCGAG184750.0130.288443
CCCGTCG129150.0130.240435
TAATCGG204300.0130.206185
CCAGTCG306250.0129.947954
TCGCGAT122250.0129.689218
GATCGAT186200.0129.61921
AACCGCG138500.0129.523031
Produced by FastQC (version 0.11.5)