FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27089623
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA97050.0131.438351
GTCGCGT96150.0130.210658
GGCCGAT261250.0129.470141
GTCGTAC175950.0129.167059
TAGCGCG67200.0129.022497
GCCGATA170950.0128.377612
CGGTCGC96400.0128.074056
CCGATAG171650.0127.0093153
CGTCGAC55350.0126.264986
ATCCGCG45500.0125.072477
GCGGTCG98450.0124.898165
GGTCGCG99400.0124.421697
CGGATCG82700.0124.225374
CTTCGAC185150.0124.16416
AATCGGT191400.0123.9513551
TCGACAA285300.0122.856518
AGGTCCG187600.0122.8444441
GATCGAT159500.0122.3423841
GATCGTA167350.0121.7055361
ACGCGTA57550.0121.318474
Produced by FastQC (version 0.11.5)