FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23795039
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGCG139250.0137.769352
CGCCGAA131100.0137.374742
AACCGCG166750.0136.937261
TTACGCG83650.0136.494789
TACGCGA112750.0136.179219
CGGGTCG278050.0136.08884
TCGGTCG127500.0135.737644
ACGATCG155300.0135.57614
CGCGATA132500.0135.430772
GTACGCG118200.0135.052068
ACGCCGA137800.0134.725511
GCCGCGT188750.0134.328053
CGGTCGG128400.0133.769795
GTCGCGA86900.0133.642387
CGTACGT115850.0133.473887
GCGGTCG121200.0133.042432
CGTTACG86000.0133.017857
ACGTACG116350.0132.970416
CGCAACA369300.0132.893342
CGCGTGA187200.0132.651675
Produced by FastQC (version 0.11.5)