FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23795039
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACG159100.0125.388344
ACGACGC159750.0125.01435
CGACGCT161700.0123.182536
CTACACG177500.0113.062841
ACACGAC178200.0112.1929243
GACGCTC181400.0109.964777
TACACGA182350.0109.842522
ACGCTCT191100.0104.232038
CGCTCTT209950.094.735839
CTTCCGA211450.019.5263210-14
TTCCGAT211350.019.48754310-14
TCCGATC204500.019.22544915-19
TCTTCCG217950.018.87081310-14
CCGATCT230850.017.37010415-19
CGAACGT14150.015.8798977
CGATCTA69900.015.07777715-19
CGACGTA11250.014.1747779
TACGACG10800.014.0941217
CGCGCAT11250.013.6720751
ACGAACG16600.013.5361776
Produced by FastQC (version 0.11.5)