FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25786956
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACG180900.0127.990114
ACGACGC180950.0127.714385
CGACGCT183450.0125.1324546
CTACACG198000.0118.0772251
GACGCTC197650.0116.0688557
ACACGAC200300.0115.919383
TACACGA209400.0111.372512
ACGCTCT211950.0108.375478
CGCTCTT231050.099.322959
TCCGATC222450.020.20924815-19
CTTCCGA234750.020.02165410-14
TCTTCCG238100.019.64253410-14
TTCCGAT245900.018.9487210-14
CCGATCT257900.017.60561415-19
CGGTACG8059.640644E-1114.4080144
CGATCTA75300.013.88100915-19
CGATCTG89950.012.796761515-19
GCTCTTC383350.012.47989810-14
TACGCGC6504.050871E-612.2664287
CGCCGTA277400.011.96323360-64
Produced by FastQC (version 0.11.5)