FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40044890
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG111950.0124.882221
TTACGCG95800.0121.303299
CGGACGT123200.0121.296541
ACGCCGA148550.0120.441731
GCGCAAC184150.0120.337841
TACGGGC262500.0119.750011
CGCCGAC103250.0118.741662
TCGGTCG79750.0118.548622
CGTTACG47800.0117.8413547
TTGCGCG61200.0117.756422
CCTTACG162650.0116.954281
CTTACCG163600.0115.784531
TCCGCGA67400.0114.872478
AACGTCG56700.0114.3152165
TTCGGTC193750.0114.2246551
GACGTTA179400.0113.152421
CGCGGTA74300.0113.133921
TTTGCGC153900.0112.841151
GTTACGC105000.0111.158128
GTCGCCG82300.0109.4022759
Produced by FastQC (version 0.11.5)