FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40044890
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC93700.0112.024815
CGACGCT94550.0109.705036
CACGACG96700.0108.699324
GACGCTC119550.087.915677
ACACGAC128250.082.354523
CTACACG133250.079.8140261
TACACGA133850.079.453752
ACGCTCT144050.072.108018
CGCTCTT174300.059.552289
CGCCGTA760600.019.7932760-64
TCGCCGT767450.019.52970360-64
GCCGTAT774900.019.33445760-64
CGTATCA780500.019.11586665-69
GTCGCCG786250.019.06826260-64
TGGTCGC809450.018.58958655-59
CCGTATC779050.018.30655765-69
GTGGTCG828050.018.22979755-59
CCGATCT155150.017.8923451
GGTCGCC807400.017.79663760-64
TCTCGGT885350.017.2185350-54
Produced by FastQC (version 0.11.5)