FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40553661
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTA131200.0124.2642751
CGATTCG99750.0123.416934
CGTATCG55850.0121.507384
CGATCGA106950.0119.718125
GACACGC252950.0117.712471
GACGCGT87100.0116.4645161
CGTCGAC61200.0116.322277
CGCGATT116350.0115.155722
CGGACGT90600.0114.4623951
GACGGCT230600.0113.550771
CCGATCG63850.0113.2098544
TCCGCGA68850.0112.768648
TCGTTCG73900.0111.242688
ACGATAC139850.0111.046821
GGCCGAT160000.0107.552561
ACCGTAA192750.0107.224941
GCGACGT110650.0106.410523
TACTCGC156750.0106.011371
CAGCGAC252550.0105.704461
CGTCGCG41650.0104.259277
Produced by FastQC (version 0.11.5)