FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32091613
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGA156000.0130.117618
ACGCGAT157400.0128.131249
CGCGGTA123200.0127.074291
TCCGCGA87850.0126.0085148
ACGGTCG70050.0125.85824
GTTCGGG269200.0125.502731
TTACGCG39700.0125.266879
CATATCG201350.0125.23744
ATCCGCG77750.0123.636237
GTCGACG51200.0121.767427
TCGACGA59950.0120.68248
GTCCGAC88800.0119.517297
TTCGGGC186300.0119.125992
GGCGACT148350.0118.716421
CGGTTAA196450.0118.62821
CTTACCG141250.0117.036371
TCGTCCG54350.0116.316646
CGCCGAT44700.0116.296782
CGTAACG49450.0115.814637
ACGCCGA69950.0115.40071
Produced by FastQC (version 0.11.5)