FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32091613
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC108400.0120.773615
CGACGCT109100.0118.5258566
CACGACG111650.0117.387894
GACGCTC131550.098.518937
ACACGAC137000.096.67223
CTACACG140300.095.7159961
TACACGA143150.092.978792
ACGCTCT149800.086.566068
CGCTCTT178150.072.831019
TCCGATC141350.017.88667515-19
CGCCGTA436950.017.64843460-64
TCGCCGT441200.017.34041660-64
GCCGTAT446250.017.24814660-64
TTCCGAT152950.017.19410110-14
CGTATCA449450.016.92873265-69
GTCGCCG458950.016.74558860-64
ACGCGAC16700.016.4956592
CTTCCGA160600.016.47437710-14
CCGTATC447850.016.42592665-69
TGGTCGC472650.016.33079755-59
Produced by FastQC (version 0.11.5)