FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25383946
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCGA92750.0134.514149
TACTCGC198000.0133.633591
GTACGCG99200.0132.198938
GTCGCGT104100.0130.8518
CGGACGT138250.0130.002671
CGCGTTA102800.0129.769292
CGATTCG95600.0129.683724
CCGATAG174000.0129.211073
CGGTACG51400.0129.19826
TCGTTCG100500.0128.829828
GATCGTA207300.0128.694471
CGATTGA251500.0128.244081
CGGTCCG84950.0127.67785
CGTCGAC58050.0127.635896
TAGGTCG155300.0127.622747
TCGTACG65700.0127.1192256
GGCTCGA303150.0126.493491
GGCCGAT181000.0126.067271
AACGTCG59000.0125.956064
CGACACG76450.0124.518665
Produced by FastQC (version 0.11.5)