FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25383946
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACG187850.0125.1090854
ACGACGC187800.0125.0265965
CGACGCT188550.0123.632336
ACACGAC205250.0114.891513
CTACACG209550.0113.811871
GACGCTC208450.0111.933687
TACACGA215600.0109.986062
ACGCTCT219600.0106.4824458
CGCTCTT240200.097.1094749
TTCCGAT233350.020.40910110-14
TCCGATC228400.020.27956215-19
CTTCCGA237850.020.25459310-14
TCTTCCG254800.018.84461410-14
CCGATCT261300.018.00913815-19
CGCCGTA324250.015.80155660-64
GCCGTAT330550.015.43460160-64
TCGCCGT332600.015.34818660-64
CGTATCA332150.015.29480165-69
CGATCTA70000.015.1806315-19
GTCGCCG347650.014.846395560-64
Produced by FastQC (version 0.11.5)