FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26947151
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC155950.0126.296485
CACGACG158000.0124.566064
CGACGCT161100.0121.842116
CTACACG177200.0112.322291
ACACGAC178800.0110.683313
GACGCTC179150.0109.44477
TACACGA182800.0108.464062
ACGCTCT194600.0100.681748
CGCTCTT217500.090.3151869
TCCGATC192200.020.18435515-19
TTCCGAT203050.019.46336610-14
CTTCCGA207600.019.26723710-14
TCTTCCG222000.018.0435910-14
CCGATCT228150.017.20085115-19
CGCCGTA283900.013.30813960-64
CGATCTA64000.013.02476915-19
GCCGTAT292450.012.95376760-64
TCGCCGT290000.012.9432260-64
CGTATCA291200.012.88502465-69
CGATCTG85450.012.80905315-19
Produced by FastQC (version 0.11.5)