FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25076251
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA78000.0129.948092
CGTACCG51050.0129.083027
CCGATCG53700.0128.534564
TAGTCGA155200.0127.06755
CGGCTAG198550.0126.528761
CTTACCG141600.0126.078621
TCGCGAG111050.0124.74581
CGTACGT66150.0124.275137
ACGTACG65800.0123.842096
TATCGCG53050.0123.814266
TAGGTCG114550.0123.217359
TAACGCG45000.0121.6442952
TCGTCGC44400.0119.034376
CGCAACG38950.0118.000229
GATCGAT130550.0117.965191
TCTACGG129350.0117.637828
CGCAACT119400.0117.3774952
ATCGTTA163950.0116.909837
ATCGTCG45150.0116.2620855
GGAGCGA190400.0115.7845159
Produced by FastQC (version 0.11.5)