FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25076251
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC111500.0121.903525
CGACGCT111600.0121.3916
CACGACG113250.0120.083824
GACGCTC129850.0104.218037
ACACGAC135550.0100.70263
TACACGA137250.099.828992
CTACACG138950.098.90681
ACGCTCT143300.094.740478
CGCTCTT163000.083.5573969
CGACGGT11150.021.6815781
CGGTACG7600.020.0306744
TCCGATC138450.018.91054215-19
TTCCGAT145800.018.67346410-14
CTTCCGA151950.018.0894110-14
CGCGAAA12600.018.023661
CGCCGTA299650.017.20053360-64
TCGCCGT305750.016.7435660-64
GCCGTAT307700.016.67514660-64
TCTTCCG165700.016.5620810-14
CCGATCT161500.016.43595515-19
Produced by FastQC (version 0.11.5)