FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005403468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005403468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26314259
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG185650.0136.749059
GACGCGT133700.0135.861181
ACGCCGA154350.0135.557311
TGTCGCG109850.0134.825327
TAGCGCG113150.0134.737327
CGCCGAA150100.0134.1362
GTCCGCG155350.0133.97547
GCGTTCG122850.0133.49584
TACTCGC161050.0132.410161
TCGCGCA128350.0132.294333
CGGACGT113300.0131.595281
AATCGGT257700.0130.808321
TCCGCGG160750.0129.88078
CGATCGG100250.0129.871031
ACGCGTT136850.0129.693082
CGGGCTA249650.0129.495932
CGTAACG115600.0129.247837
GTAACGC200350.0128.054358
CGTATCG55300.0127.946337
GCGCGTC119300.0127.60929
Produced by FastQC (version 0.11.5)